HPLC technique was applied to quantitatively analyze ketoconazole in nanostructured lipid carrier. The chromatographic method was performed by using an isocratic system. The mobile phase was composed of acetonitrile-methanol in the ratio of (70:30%) with a flow rate of 1 ml/min; the detection wavelength was at 295 nm. The standard calibration curve was linear over the concentration range of 50-300 μg/ml with R2 (0.997) and ketoconazole was successfully separated with good linearity (the regression equation is Y = 49968x+10872 (R² = 0.997). The limit of detection (LOD) and limit of quantitation (LOQ) obtained for ketoconazole were 0.001040664 μg/ml and 0.003153526 μg/ml. The HPLC technique was proper for quality control of ketoconazole in nanostructured lipid carrier dispersion.
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